This biotin-conjugated mouse monoclonal antibody (clone 7A8) targets rhesus macaque IgG4, exhibiting high specificity for the IgG4 subclass with minimal cross-reactivity to rhesus IgG1, IgG2, or IgG3. It is particularly suited for applications requiring selective detection or quantification of rhesus IgG4 in the presence of other IgG subclasses.
This reagent is a valuable tool for studies of isotype-specific immune responses in rhesus macaques, including vaccine evaluation, allergen-specific responses, and Fc receptor engagement assays. The biotin label enables versatile detection using streptavidin-conjugated fluorophores or enzymes, facilitating its use in flow cytometry, ELISA, or immunoassays—although users should validate suitability for their specific application.
Anti-rhesus IgG4 [tessera] was developed and validated for in vitro research use, and is available through NIAID sponsored activities.
Key Features
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Highly specific for rhesus IgG4
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Minimal cross-reactivity with rhesus IgG1, IgG2, IgG3
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Biotin-conjugated for flexible assay detection
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Suitable for flow cytometry, ELISA, and other biotin-avidin based immunoassays
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Validated in nonhuman primate samples
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Intended for in vitro use only
Suggested Applications
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Monitoring IgG4 subclass responses in rhesus macaques
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Immunoprofiling in vaccine or allergen studies
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Fcγ receptor binding studies specific to IgG4
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Use in multiplex detection panels with other subclass-specific reagents
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